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| Geslin, Claire; Gaillard, Melusine; Flament, Didier; Rouault, Karen; Le Romancer, Marc; Prieur, Daniel; Erauso, Gael. |
| Only one virus-like particle (VLP) has been reported from hyperthermophilic Euryarchaeotes. This VLP, named PAV1 is shaped like a lemon and was isolated from a strain of "Pyrococcus abyssi," a deep-sea isolate. Its genome consists of a double-stranded circular DNA of 18 kb which is also present at a high copy number (60 per chromosome) free within the host cytoplasm but is not integrated into the host chromosome. Here, we report the results of complete analysis of the PAV1 genome. All the 25 predicted genes, except 3, are located on one DNA strand. A transcription map has been made by using a reverse transcription-PCR assay. All the identified open reading frames (ORFs) are transcribed. The most significant similarities relate to four ORFs. ORF 180a shows... |
| Tipo: Text |
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| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-3536.pdf |
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| Krupovic, Mart; Gonnet, Mathieu; Ben Hania, Wajdi; Forterre, Patrick; Erauso, Gael. |
| Mobilome of hyperthermophilic archaea dwelling in deep-sea hydrothermal vents is poorly characterized. To gain insight into genetic diversity and dynamics of mobile genetic elements in these environments we have sequenced five new plasmids from different Thermococcus strains that have been isolated from geographically remote hydrothermal vents. The plasmids were ascribed to two subfamilies, pTN2-like and pEXT9a-like. Gene content and phylogenetic analyses illuminated a robust connection between pTN2-like plasmids and Pyrococcus abyssi virus 1 (PAV1), with roughly half of the viral genome being composed of genes that have homologues in plasmids. Unexpectedly, pEXT9a-like plasmids were found to be closely related to the previously sequenced plasmid pMETVU01... |
| Tipo: Text |
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| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00125/23583/21434.pdf |
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| Mei, Nan; Postec, Anne; Monnin, Christophe; Pelletier, Bernard; Payri, Claude E.; Menez, Benedicte; Frouin, Eleonore; Ollivier, Bernard; Erauso, Gael; Quemeneur, Marianne. |
| High amounts of hydrogen are emitted in the serpentinite-hosted hydrothermal field of the Prony Bay (PHF, New Caledonia), where high-pH (similar to 11), low-temperature (< 40 degrees C), and low-salinity fluids are discharged in both intertidal and shallow submarine environments. In this study, we investigated the diversity and distribution of potentially hydrogen-producing bacteria in Prony hyperalkaline springs by using metagenomic analyses and different PCR-amplified DNA sequencing methods. The retrieved sequences of hydA genes, encoding the catalytic subunit of [FeFe]-hydrogenases and, used as a molecular marker of hydrogen-producing bacteria, were mainly related to those of Firmicutes and clustered into two distinct groups depending on sampling... |
| Tipo: Text |
Palavras-chave: Hydrogen; Microbial diversity; Hydrogen producers; Serpentinization; HydA genes; [FeFe]-hydrogenase; Metagenomics. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00467/57913/60308.pdf |
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| Postec, Anne; Quemeneur, Marianne; Bes, Meline; Mei, Nan; Benaissa, Fatma; Payri, Claude; Pelletier, Bernard; Monnin, Christophe; Guentas-dombrowsky, Linda; Ollivier, Bernard; Gerard, Emmanuelle; Pisapia, Celine; Gerard, Martine; Menez, Benedicte; Erauso, Gael. |
| Active carbonate chimneys from the shallow marine serpentinizing Prony Hydrothermal Field were sampled 3 times over a 6 years period at site ST09. Archaeal and bacterial communities composition was investigated using PCR-based methods (clone libraries, Denaturating Gel Gradient Electrophoresis, quantitative PCR) targeting 16S rRNA genes, methyl coenzyme M reductase A and dissimilatory sulfite reductase subunit B genes. Methanosarcinales (Euryarchaeota) and Thaumarchaea were the main archaeal members. The Methanosarcinales, also observed by epifluorescent microscopy and FISH, consisted of two phylotypes that were previously solely detected in two other serpentinitzing ecosystems (The Cedars and Lost City Hydrothermal Field). Surprisingly, members of the... |
| Tipo: Text |
Palavras-chave: Hydrothermal; Serpentinization; Alkaline; Prony; Microbial communities. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00467/57916/60307.pdf |
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| Pisapia, Celine; Gerard, Emmanuelle; Gerard, Martine; Lecourt, Lena; Lang, Susan Q.; Pelletier, Bernard; Payri, Claude E.; Monnin, Christophe; Guentas, Linda; Postec, Anne; Quemeneur, Marianne; Erauso, Gael; Menez, Benedicte. |
| Despite their potential importance as analogs of primitive microbial metabolisms, the knowledge of the structure and functioning of the deep ecosystems associated with serpentinizing environments is hampered by the lack of accessibility to relevant systems. These hyperalkaline environments are depleted in dissolved inorganic carbon (DIC), making the carbon sources and assimilation pathways in the associated ecosystems highly enigmatic. The Prony Bay Hydrothermal Field (PHF) is an active serpentinization site where, similar to Lost City (Mid-Atlantic Ridge), high-pH fluids rich in H-2 and CH4 are discharged from carbonate chimneys at the seafloor, but in a shallower lagoonal environment. This study aimed to characterize the subsurface microbial ecology of... |
| Tipo: Text |
Palavras-chave: Serpentinization; Alkaline hydrothermalism; Deep life; Firmicutes; Acetothermia; Omnitrophica; Organic carbon. |
| Ano: 2017 |
URL: https://archimer.ifremer.fr/doc/00467/57909/60314.pdf |
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| Mei, Nan; Postec, Anne; Erauso, Gael; Joseph, Manon; Pelletier, Bernard; Payri, Claude; Ollivier, Bernard; Quemeneur, Marianne. |
| A novel anaerobic, alkaliphilic, Gram-stain-positive, spore-forming bacterium was isolated from a carbonaceous hydrothermal chimney in Prony Bay, New Caledonia. This bacterium, designated strain 3b(T), grew at temperatures from 30 to 43 degrees C (optimum 37 degrees C) and at pH between 7.8 and 10.1 (optimum 9.5). Added NaCl was not required for growth (optimum 0-0.2 %, w/v), but was tolerated at up to 4 %. Yeast extract was required for growth. Strain 3b(T) utilized crotonate, lactate and pyruvate, but not sugars. Crotonate was dismutated to acetate and butyrate. Lactate was disproportionated to acetate and propionate. Pyruvate was degraded to acetate plus trace amounts of hydrogen. Growth on lactate was improved by the addition of fumarate, which was... |
| Tipo: Text |
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| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00467/57912/60312.pdf |
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